Wu Z, Li J, Zhang T, Zhang K, Liu X, Yang Z, Xu L,. One-pot synthesized three-way junction based multiple strand displacement amplification for sensitive assay of H5N1 DNA. Analyst. 2025 Mar 13. Abstract submitted by kickingbird at Mar, 14, 2025 from Analyst. 2025 Mar 13 (via https://pubs.rsc.org/en/content/articlelanding/2025/an/d4an0) The rapid and sensitive detection of H5N1, a highly pathogenic avian influenza virus, is crucial for controlling its spread and minimizing its impact on public health. In this study, we developed a novel ... Azeem S, Yoon KJ. Diagnostic Assays for Avian Influenza Virus Surveillance and Monitoring in Poultry. Viruses. 2025 Feb 6;17(2):228. Abstract submitted by kickingbird at Feb, 27, 2025 from Viruses. 2025 Feb 6;17(2):228 (via https://www.mdpi.com/1999-4915/17/2/228) Diagnostic testing plays a key role in a surveillance program as diagnostic testing aims to accurately determine the infection or disease status of an individual animal. Diagnostic assays for AIV can be ... Pan X, Zhou F, Shi X, Liu Q, Yan D, Teng Q, Yuan C. The Generation of a H9N2 Avian Influenza Virus with HA and C3d-P29 Protein Fusions and Vaccine Development Applications. Vaccines (Basel). 2025 Jan 21;13(2):99. Abstract submitted by kickingbird at Feb, 27, 2025 from Vaccines (Basel). 2025 Jan 21;13(2):99 (via https://www.mdpi.com/2076-393X/13/2/99) Background: Maternal-derived antibody (MDA) interferes with immune responses, leading to the failure of H9N2 avian influenza vaccinations in poultry. So far, none of the commercially available H9N2 avian ... Ye Q, Xiao Z, Bai C, Yao H, Zhao L, Tan WS. Unveiling the multi-characteristic potential of hyper-productive suspension MDCK cells for advanced influenza A virus propagation. Vaccine. 2025 Feb 21;52:126900. Abstract submitted by kickingbird at Feb, 24, 2025 from Vaccine. 2025 Feb 21;52:126900 (via https://pubmed.ncbi.nlm.nih.gov/39985968/) The global population faces persistent threats from influenza viruses, with vaccination remaining the most cost-effective preventive measure against influenza. Mammalian cell-based influenza vaccine production ... Qila Sa, etc.,al. A programmable nucleic acid fluorescence biosensor based on the BstNI endonuclease for detection of the influenza A (H1N1) virus. Biomolecular Spectroscopy, 2025. Abstract submitted by kickingbird at Feb, 22, 2025 from Biomolecular Spectroscopy, 2025 (via https://www.sciencedirect.com/science/article/abs/pii/S13861) Timely and accurate diagnosis of influenza virus is essential to prevent the spread of disease and to select an appropriate treatment strategy. Here, we report the development of a novel fluorescence biosensor ... Kumar J, Xu M, Li YA, You SW, Doherty BM, Gardiner. Capacitive Biosensor for Rapid Detection of Avian (H5N1) Influenza and E. coli in Aerosols. ACS Sens. 2025 Feb 21. Abstract submitted by kickingbird at Feb, 22, 2025 from ACS Sens. 2025 Feb 21 (via https://pubs.acs.org/doi/10.1021/acssensors.4c03087) Airborne transmission via aerosols is a dominant route for the transmission of respiratory pathogens, including avian H5N1 influenza A virus and E. coli bacteria. Rapid and direct detection of respiratory ... Kilany WH, Safwat M, Zain El-Abideen MA, Hisham I,. Multivalent Inactivated Vaccine Protects Chickens from Distinct Clades of Highly Pathogenic Avian Influenza Subtypes H5N1 and H5N8. Vaccines. 2025; 13(2):204. Abstract submitted by kickingbird at Feb, 21, 2025 from Vaccines. 2025; 13(2):204 (via https://www.mdpi.com/2076-393X/13/2/204) Background/Objective: Highly pathogenic avian influenza (HPAI) H5 subtype remains a significant menace to both the poultry industry and human public health. Biosecurity and mass vaccination of susceptible ... Zhou S, Zhang Y, Liu S, Peng C, Shang J, Tian J, L. Pathogenicity of Novel H3 Avian Influenza Viruses in Chickens and Development of a Promising Vaccine. Viruses. 2025; 17(3):288. Abstract submitted by kickingbird at Feb, 21, 2025 from Viruses. 2025; 17(3):288 (via https://www.mdpi.com/1999-4915/17/3/288) Since 2022, three cases of human infections of novel H3N8 avian influenza viruses (AIVs) have been confirmed in China. Given the potential for significant public health implications, the prompt detection ... Wang L, Bu S, Meng H, Zhang X, Zhou H, Xu C, Hao Z. Lateral flow biosensor development for the visual identification of H1N1 virus based on primer extension nucleic acid isothermal amplification and M13mp18 single-stranded DNA. Mikrochim Acta. 2025 Feb 18;192(3):171. Abstract submitted by kickingbird at Feb, 20, 2025 from Mikrochim Acta. 2025 Feb 18;192(3):171 (via https://link.springer.com/article/10.1007/s00604-025-07031-1) A lateral flow biosensor was developed based on newly established primer extension nucleic acid isothermal amplification (PEIA) and M13mp18 single-stranded DNA (ssDNA) to visually identify the H1N1 virus. ... Ibrahim S, Spackman E, Suarez DL, Goraichuk IV, Le. Evaluation of an N1 NA antibody-specific enzyme-linked lectin assay for detection of H5N1 highly pathogenic avian influenza virus infection in vaccinated birds. J Virol Methods. 2025 Feb 14:115127. Abstract submitted by kickingbird at Feb, 18, 2025 from J Virol Methods. 2025 Feb 14:115127 (via https://www.sciencedirect.com/science/article/pii/S016609342) Unprecedented H5N1 highly pathogenic avian influenza (HPAI) outbreaks are occurring around the world and there is growing interest in the use of vaccines in affected regions. Vaccination when properly ... Liu J, An T, Peng J, Zhu Q, Zhao H, Liang Z, Mo K,. An amplification-free digital droplet assay for influenza A viral RNA based on CRISPR/Cas13a. Analyst. 2025 Feb 14. Abstract submitted by kickingbird at Feb, 15, 2025 from Analyst. 2025 Feb 14 (via https://pubs.rsc.org/en/content/articlelanding/2025/an/d4an0) Most of the CRISPR-based RNA detection methods are combined with amplification to improve sensitivity, which lead to some drawbacks such as aerosol pollution, complicated operation, and amplification bias. ... Wang X, Dai J, Yang W, Yao Y, Zhang J, Liu K, Lu X. Spray vaccination with a safe and bivalent H9N2 recombinant chimeric NDV vector vaccine elicits complete protection against NDV and H9N2 AIV challenge. Vet Res. 2025 Jan 31;56(1):24. Abstract submitted by kickingbird at Feb, 3, 2025 from Vet Res. 2025 Jan 31;56(1):24 (via https://veterinaryresearch.biomedcentral.com/articles/10.118) Newcastle disease virus (NDV) and H9N2 avian influenza virus (AIV) represent significant pathogenic risks to the poultry industry, leading to considerable economic losses. Vaccination is a widely used ... Wang Z, Tian C, Zhu J, Wang S, Ao X, He Y, Chen H,. Avian influenza mRNA vaccine encoding hemagglutinin provides complete protection against divergent H5N1 viruses in specific-pathogen-free chickens. J Nanobiotechnology. 2025 Jan 29;23(1):55. Abstract submitted by kickingbird at Jan, 30, 2025 from J Nanobiotechnology. 2025 Jan 29;23(1):55 (via https://jnanobiotechnology.biomedcentral.com/articles/10.118) Background: The rapid mutation of avian influenza virus (AIV) poses a significant threat to both the poultry industry and public health. Herein, we have successfully developed an mRNA-LNPs candidate vaccine ... Zhang C, Hou J, Li Z, Shen Q, Bai H, Chen L, Shen. PROTAR Vaccine 2.0 generates influenza vaccines by degrading multiple viral proteins. Nat Chem Biol. 2025 Jan 15. Abstract submitted by kickingbird at Jan, 17, 2025 from Nat Chem Biol. 2025 Jan 15 (via https://www.nature.com/articles/s41589-024-01813-z) Manipulating viral protein stability using the cellular ubiquitin-proteasome system (UPS) represents a promising approach for developing live-attenuated vaccines. The first-generation proteolysis-targeting ... Shen J, Li J, Shen Q, Hou J, Zhang C, Bai H, Ai X,. Proteolysis-targeting influenza vaccine strains induce broad-spectrum immunity and in vivo protection. Nat Microbiol. 2025 Jan 15. Abstract submitted by kickingbird at Jan, 17, 2025 from Nat Microbiol. 2025 Jan 15 (via https://www.nature.com/articles/s41564-024-01908-2) Generating effective live vaccines from intact viruses remains challenging owing to considerations of safety and immunogenicity. Approaches that can be applied in a systematic manner are needed. Here we ... Gert Zimmer, etc.,al. [preprint]Immunization with a novel RNA replicon vaccine confers long-lasting protection against H5N1 avian influenza virus in 24 bird species. ttps://doi.org/10.1101/2025.01.15.633174. Abstract submitted by kickingbird at Jan, 16, 2025 from ttps://doi.org/10.1101/2025.01.15.633174 (via https://www.biorxiv.org/content/10.1101/2025.01.15.633174v1) Highly pathogenic avian influenza viruses (HPAIV) of subtype H5N1 (clade 2.3.4.4b) have spread worldwide and caused the death of hundreds of millions of wild birds and domestic poultry. Moreover, spill ... Bia P, Losardo M, Manna A, Brusaferro S, Privitera. Selected microwave irradiation effectively inactivates airborne avian influenza A(H5N1) virus. Sci Rep. 2025 Jan 15;15(1):2021. Abstract submitted by kickingbird at Jan, 16, 2025 from Sci Rep. 2025 Jan 15;15(1):2021 (via https://www.nature.com/articles/s41598-025-85376-6) The highly pathogenic avian influenza A(H5N1) virus threatens animal and human health globally. Innovative strategies are crucial for mitigating risks associated with airborne transmission and preventing ... Qingcui Wang, Biru Yang, Yaqi Zhu, Jiameng Pei, et. Trans-dimensional nanocoral gold foam interfaces affords ultrasensitive detection of influenza virus. Anal Chim Acta . 2025 Feb 1:1337:343576.. Abstract submitted by kickingbird at Jan, 14, 2025 from Anal Chim Acta . 2025 Feb 1:1337:343576. (via https://www.sciencedirect.com/science/article/abs/pii/S00032) Development of sensitive and cost-effective strategies for detecting influenza viruses is crucial to combat the spread of infectious diseases. In this study, a novel trans-dimensional nanocoral gold foam ... Jinha Song, etc.,al. Rapid and specific on-site H5Nx avian influenza diagnosis via RPA and PAM-independent CRISPR-Cas12a assay combined with anti-NP antibody-based viral RNA purification. Front. Vet. Sci. Abstract submitted by kickingbird at Jan, 10, 2025 from Front. Vet. Sci (via https://www.frontiersin.org/journals/veterinary-science/arti) Rapid and accurate detection of H5Nx avian influenza viruses is critical for effective surveillance and control measures. Currently, RT-qPCR with spin column RNA extraction is the gold standard for HPAIV ... Ashraf MA, Raza MA, Imran A, Amjad MN. Next-generation vaccines for influenza B virus: advancements and challenges. Arch Virol. 2025 Jan 6;170(2):25. Abstract submitted by kickingbird at Jan, 8, 2025 from Arch Virol. 2025 Jan 6;170(2):25 (via https://link.springer.com/article/10.1007/s00705-024-06210-4) To battle seasonal outbreaks of influenza B virus infection, which continue to pose a major threat to world health, new and improved vaccines are urgently needed. 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