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2025-2-9 13:37:07
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DCA Moraes, etc.,al. Assessment of individual and population-based sampling for detection of Influenza A virus RNA in breeding swine herds. Veterinary Microbiology.  Abstract  
submitted by kickingbird at 6 hours ago from Veterinary Microbiology (via https://www.sciencedirect.com/science/article/abs/pii/S03781)
Sample types currently used for Influenza A virus (IAV) surveillance in swine farms vary in sensitivity, convenience of collection, and herd representativeness. Family oral fluids are an effective population-based ...

Abousenna MS, Shafik NG, Abotaleb MM. Evaluation of humoral immune response and milk antibody transfer in calves and lactating cows vaccinated with inactivated H5 avian influenza vaccine. Sci Rep. 2025 Feb 7;15(1):4637.  Abstract  
submitted by kickingbird at 6 hours ago from Sci Rep. 2025 Feb 7;15(1):4637 (via https://www.nature.com/articles/s41598-025-87831-w)
The detection of Highly Pathogenic Avian Influenza (HPAI) A(H5N1) in dairy cattle in the United States has raised concerns about human exposure. This study evaluated the efficacy of various doses of an ...

Richard Webby, Thomas Fabrizio, Ahmed Kandeil et al. [preprint]Influenza A(H5N1) Viruses Isolated From Dairy Cattle Demonstrate High Virulence in Laboratory Models, but Retain Avian Virus-like Properties. https://doi.org/10.21203/rs.3.rs-5806806/v1.  Abstract  
submitted by kickingbird at 1 days ago from https://doi.org/10.21203/rs.3.rs-5806806/v1 (via https://www.researchsquare.com/article/rs-5806806/v1)
In March 2024, clade 2.3.4.4b highly pathogenic avian influenza A(H5N1) viruses were first detected in U.S. dairy cattle. Similar viruses have since caused 66 zoonotic human infections. To assess changes ...

Griffiths ME, Broos A, Morales J, Tu IT, Bergner L. Dynamics of influenza transmission in vampire bats revealed by longitudinal monitoring and a large-scale anthropogenic perturbation. Sci Adv. 2025 Feb 7;11(6):eads1267.  Abstract  
submitted by kickingbird at 3 days ago from Sci Adv. 2025 Feb 7;11(6):eads1267 (via https://www.science.org/doi/10.1126/sciadv.ads1267)
Interrupting pathogen transmission between species is a priority strategy to mitigate zoonotic threats. However, avoiding counterproductive interventions requires knowing animal reservoirs of infection ...

Harvey J, Sullivan JD, Poulson RL, Carter DL, Dris. Highly Pathogenic Avian Influenza Virus H5N1 in Double-crested Cormorants (Nannopterum auritum) of the Chesapeake Bay, USA. J Wildl Dis. 2025 Feb 6.  Abstract  
submitted by kickingbird at 3 days ago from J Wildl Dis. 2025 Feb 6 (via https://pubmed.ncbi.nlm.nih.gov/39911059/)
Double-crested Cormorants (Nannopterum auritum) have historically exhibited low levels of infection and antibodies to avian influenza virus (AIV). The recent global expansion of clade 2.3.4.4b A/goose/Guangdong/1/1996 ...

Oliver Eales, James M McCaw, Freya M Shearer. [preprint]Viral kinetics of H5N1 infections in dairy cattle. https://doi.org/10.1101/2025.02.01.636082.  Abstract  
submitted by kickingbird at 3 days ago from https://doi.org/10.1101/2025.02.01.636082 (via https://www.biorxiv.org/content/10.1101/2025.02.01.636082v1)
Since early-2024 unprecedented outbreaks of highly pathogenic avian influenza H5N1 clade 2.3.4.4b have been ongoing in dairy cattle in the United States with significant consequences for the dairy industry ...

Weibo Tang, etc.,al. Rapid aging of influenza epidemics in China from 2005/06 to 2016/17: a population-based study. Infectious Disease Modelling.  Abstract  
submitted by kickingbird at 3 days ago from Infectious Disease Modelling (via https://www.sciencedirect.com/science/article/pii/S246804272)
BackgroundChina is an aging society, and the older population is at a higher risk of influenza infection and influenza-related mortality. However, there is limited knowledge regarding the aging of influenza ...

Mahmoud SH, Khattab MS, Yehia N, Zanaty A, Arafa A. Pathogenicity of Highly Pathogenic Avian Influenza A/H5Nx Viruses in Avian and Murine Models. Pathogens. 2025; 14(2):149.  Abstract  
submitted by kickingbird at 4 days ago from Pathogens. 2025; 14(2):149 (via https://www.mdpi.com/2076-0817/14/2/149)
The evolution and adaptation of highly pathogenic avian influenza (HPAI) viruses pose ongoing challenges for animal and public health. We investigated the pathogenic characteristics of the newly emerged ...

Hannah L. Wallace, etc.,al. [preprint]No Evidence of Anti-influenza Nucleoprotein Antibodies in Retail Milk from Across Canada (April - July 2024). https://doi.org/10.1101/2025.01.31.25321461.  Abstract  
submitted by kickingbird at 4 days ago from https://doi.org/10.1101/2025.01.31.25321461 (via https://www.medrxiv.org/content/10.1101/2025.01.31.25321461v)
Following reports of HPAI H5N1 infections of dairy cattle in the United States (US) in March 2024, we established a Pan-Canadian Milk network to monitor retail milk in Canada. Milk samples from across ...

Artois J, Vergne T, Fourtune L, Dellicour S, Scoiz. Spatial risk modelling of highly pathogenic avian influenza in France: Fattening duck farm activity matters. PLoS One. 2025 Feb 4;20(2):e0316248.  Abstract  
submitted by kickingbird at 4 days ago from PLoS One. 2025 Feb 4;20(2):e0316248 (via https://journals.plos.org/plosone/article?id=10.1371/journal)
In this study, we present a comprehensive analysis of the key spatial risk factors and predictive risk maps for HPAI infection in France, with a focus on the 2016-17 and 2020-21 epidemic waves. Our findings ...

Hirst JC, Hutchinson EC. Purification of Influenza Virions. Methods Mol Biol. 2025;2890:27-51.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:27-51 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
This chapter describes basic workflows for concentrating and for purifying influenza virions. It presents several ways in which ultracentrifugation can be used to concentrate influenza virions from the ...

Zhou J, Hemmink JD, Whittaker CJ, Shelton HA, Peac. Animal Models in Influenza Research. Methods Mol Biol. 2025;2890:53-88.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:53-88 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Animal model systems for human and animal influenza virus infection and transmission have been established to address research questions which cannot be addressed using in vitro models. Several animal ...

Broich L, Fu Y, Sieben C. Live-Cell Single-Molecule Imaging of Influenza A Virus-Receptor Interaction. Methods Mol Biol. 2025;2890:89-101.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:89-101 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A viruses are a major health care burden, and their biology has been intensely studied for decades. However, many details of virus infection are still elusive, requiring the development of refined ...

Pathak T, Gupta K, Kumar N, Banerjee I. Monitoring Influenza A Virus Entry Using Quantitative Fluorescence Microscopy. Methods Mol Biol. 2025;2890:103-123.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:103-123 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A virus (IAV) is a major threat to global human health and is a topic of intense research. With the continuous problem of seasonal influenza and the threat of potential pandemics due to frequent ...

Nakano M, Noda T. Purification and Ultramicroscopic Observation of the Influenza A Virus Ribonucleoprotein Complex. Methods Mol Biol. 2025;2890:141-149.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:141-149 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A virus (IAV) has an eight-segmented, single-stranded, negative-sense viral genomic RNA (vRNA). Each vRNA strand associates with nucleoproteins and an RNA-dependent RNA polymerase complex to ...

Peterl S, Wachsmuth-Melm M, Chlanda P. Sample Preparation for Cryo-Electron Tomography of Influenza A Virus and Infected Cells. Methods Mol Biol. 2025;2890:169-184.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:169-184 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Influenza A virus (IAV) replication within host cells relies on tightly controlled, dynamic interactions between viral and cellular components. Yet we have a limited understanding of the viral replication ...

Sousa AL, Tranfield EM. Room Temperature Transmission Electron Microscopy of Influenza A Virus Samples. Methods Mol Biol. 2025;2890:151-167.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:151-167 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
Transmission electron microscopy is an imaging technique that visualizes the ultrastructure of samples, revealing not just the structures of interest but also the context occurring around the structures ...

Lee N. Mapping Binding Sites of Nucleoprotein Within the Influenza Virus RNA Genome. Methods Mol Biol. 2025;2890:211-224.  Abstract  
submitted by kickingbird at 6 days ago from Methods Mol Biol. 2025;2890:211-224 (via https://link.springer.com/protocol/10.1007/978-1-0716-4326-6)
With the advent of next-generation sequencing, a plethora of techniques have been developed to uncover nucleic acid interactions with unprecedented resolution. For example, UV-crosslinking and immunoprecipitation ...

Cha RM, Park MJ, Baek YG, Lee YN, Jang Y, Kang YM,. Genetic characteristics and pathogenesis of clade 2.3.4.4b H5N1 high pathogenicity avian influenza virus isolated from poultry in South Korea, 2022-2023. Virus Res. 2025 Jan 31:199541.  Abstract  
submitted by kickingbird at 6 days ago from Virus Res. 2025 Jan 31:199541 (via https://pubmed.ncbi.nlm.nih.gov/39894372/)
During the 2022-2023 winter season in South Korea, a novel clade 2.3.4.4b H5N1 HPAIV was first detected in wild birds, which then subsequently caused multiple outbreaks in poultry farms and wild birds. ...

Kirkpatrick Roubidoux E, Meliopoulos V, Livingston. Intraductal infection with H5N1 clade 2.3.4.4b influenza virus. J Virol. 2025 Jan 31:e0192724.  Abstract  
submitted by kickingbird at 8 days ago from J Virol. 2025 Jan 31:e0192724 (via https://journals.asm.org/doi/10.1128/jvi.01927-24)
In March 2024, highly pathogenic avian influenza (HPAI) H5N1 of the clade 2.3.4.4b was detected in dairy cows in Texas and has since been detected in several other U.S. states. Virus has been detected ...

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