ZHANG J, Feng Y, Hu D, Lv H, et al. Rapid and sensitive detection of H7N9 avian influenza virus using reverse transcription loop-mediated isothermal amplification. J Clin Microbiol. 2013 Sep 4
An epidemic of human H7N9 influenza virus infection has recently emerged in China, which was clinically featuring with high mortality and while also resulting in serious economic loss. The novel reassortant avian-origin influenza A (H7N9) virus, as the causative agent of this epidemic, raised the possibility of triggering a large-scale of flu pandemic worldwide. It seemed likely that fast molecular detection assays specific for this viruses would be in great demand. Here we report a one-step RT-LAMP method for rapid detection of HA gene and NA gene of H7N9 virus, the minimum detection limit of which was evaluated using in vitro transcription RNA templates. Totally, 135 samples from clinical specimens (from either patients or poultry) were subjected to testing by this method in comparison with the real time PCR recommend by the World Health Organization (WHO). Our results showed that 1) RT-LAMP-based trials can be completed in 12~23 minute, 2) detection limit for H7 gene is around 10 copies per reaction, which is similar to that of the real time PCR whereas that for its counterpart N9 gene is 5 copies per reaction with a 100-fold higher sensitivity than the WHO recommended-method. Indeed, this excellent performance of our method was also validated by a series of clinical specimens. Therefore we believe that the simple, fast and sensitive method of RT-LAMP might be widely applied in the field detection for H7N9 infections and play a role in prevention of an influenza pandemic.
See Also:
Latest articles in those days:
- [preprint]A systematic review of laboratory investigations into the pathogenesis of avian influenza viruses in wild avifauna of North America 22 hours ago
- [preprint]Seabird and sea duck mortalities were lower during the second breeding season in eastern Canada following the introduction of Highly Pathogenic Avian Influenza A H5Nx viruses 22 hours ago
- [preprint]Novel introductions of human-origin H3N2 Influenza viruses in Swine, Chile 22 hours ago
- Long term evolution of human seasonal influenza virus A(H3N2) is associated with an increase in polymerase complex activity 23 hours ago
- Humoral correlates of protection against influenza A H3N2 virus infection 23 hours ago
[Go Top] [Close Window]