Xing Z, Cardona C, Dao P, Crossley B, Hietala S, B. Realtime RT-PCR Assay Unable to Detect H7 Subtype Avian Influenza Viruses Isolated from Wild Birds. J Clin Microbiol. 2008 Mar 5
We report a failure of the current real-time RT-PCR H7 subtyping protocol, used in national avian influenza surveillance programs. Significant substitutions in primer and probe target sequences were identified, especially in wild bird viruses. The protocol, designed originally for detecting H7 influenza viruses in poultry, is not reliable for wild bird surveillance.
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