HAN Qing-gong; QIAO Chuan-ling etc.,al. Sequencing and eukaryotic expression of HA gene of H9N2 subtype influenza virus from swine. Chinese Journal of Preventive Veterinary Medicine
HA gene of SW/ZhJ7/04 was amplified by RT-PCR,sequenced,and cloned into recombinant plasmid pCAGGS-HA. The 293T cells monolayer were transfected with plasmid pCAGGS-HA,expression of the HA protein was detected by SDS-PAGE 48 h after transfection and its immunogenic activity was confirmed by Western blot assay.The immunogenicity of the plasmid pCAGGS-HA was evaluated in mice.
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