Ng SS, Li OT, Cheung TK, Peiris JM, Poon LL. Heterologous influenza vRNA segments with identical non-coding sequences stimulate viral RNA replication in trans. Virol J. 2008 Jan 11;5(1):2
ABSTRACT: The initiation of transcription and replication of influenza A virus requires the 5´ and 3´ ends of vRNA. Here, the role of segment-specific non-coding sequences of influenza A virus on viral RNA synthesis was studied. Recombinant viruses, with the nonstructural protein (NS) segment-specific non-coding sequences replaced by the corresponding sequences of the neuraminidase (NA) segment, were characterized. The NS and NA vRNA levels in cells infected with these mutants were much higher than those of the wild type, whereas the NS and NA mRNA levels of the mutants were comparable to the wild-type levels. By contrast, the PB2 vRNA and mRNA levels of all the tested viruses were similar, indicating that vRNA with heterologous segment-specific non-coding sequences was not affected by the mutations. The observations suggested that, with the cooperation between the homologous 5´ and 3´segment-specific sequences, the introduced mutations could specifically enhance the replication of NA and NS vRNA.
See Also:
Latest articles in those days:
- [preprint]A systematic review of laboratory investigations into the pathogenesis of avian influenza viruses in wild avifauna of North America 11 hours ago
- [preprint]Seabird and sea duck mortalities were lower during the second breeding season in eastern Canada following the introduction of Highly Pathogenic Avian Influenza A H5Nx viruses 11 hours ago
- [preprint]Novel introductions of human-origin H3N2 Influenza viruses in Swine, Chile 11 hours ago
- Long term evolution of human seasonal influenza virus A(H3N2) is associated with an increase in polymerase complex activity 11 hours ago
- Humoral correlates of protection against influenza A H3N2 virus infection 11 hours ago
[Go Top] [Close Window]