Objective To construct recombinant fowlpox virus (rFPV) co-expressing multi-epitopes of influenza/A subtype H3, H5, H5, H7, and H9 virus. Methods The epitope gene of dominant antigen (HA NA NP M) d of influenza A viruses was selected. The multi-epitopes gene was synthesized and optimized by bioinformatics methods, and then inserted into the H5 H7 HA gene to construct an ORF, which should be cloned into downstream of the complex prompter (ATI-P7.5×20) of pUTA-2 vector to construct the recombinant fowlpox virus...

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