To construct a recombinant vaccinia virus RVJ1175M2 expressing influenza A3 virus(H3N2) M2 gene,full length gene encoding influenza virus(H3N2) M2 protein was amplified with PCR and cloned into plasmid pJSC1175 which was used for homologous recombination with vaccinia virus Tiantan strain.Along with this,a recombinant vaccinia virus RVJ1175M2 containing the M2 gene was subsequenlty constructed.It was identified by PCR that the gene of M2 protein was inserted into the TK locus of vaccinia virus Tiantan strai...

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