The NS1 gene of the H5N1 subtype avian influenza virus was amplified by RT-PCR,and the amplified product was cloned into the eukaryotic expression vector pCMV-Myc,then it was transfected into A549 cells.After 48 h,the expression of NS1 was detected by Western blot.Fluorescence and electron microscopy and flow cytometry showed that the NS1 gene of H5N1 avian influenza virus could induce apoptosis in human pulmonary carcinoma cell line A549.

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