LIU Bin; LI Wen-ping; QU Xiao-chu; TANG Yu-long; Z. Expression of nucleoprotein of avian influenza virus bait vectors and detection of its self-activation in yeast two-hybrid system. Chinese Journal of Zoonoses
To express of nucleoprotein(NP) of avian influenza virus(H5N1) in yeast two-hybrid system and to detect its self-activation in this system,the complete encoding gene of NP was amplified from a plasmid pGEMT/H5NP by PCR and it was inserted into pGBKT7 vector according to the reading frame.The bait vectors were transformed into the yeast strain AH109 by PEG/LiAc method and its self-activation was tested by both the phenotype assay and the color assay,then expression of Gal4-NP fusion proteins identified by We...
Fulltext in Chinese can be downloaded in Chinese version of F.I.C
See Also:
Latest articles in those days:
- Influenza D Virus Infection in China, 2022-2023 5 hours ago
- Evidence of reassortment of avian influenza A (H2) viruses in Brazilian shorebirds 5 hours ago
- Epitopes in the HA and NA of H5 and H7 avian influenza viruses that are important for antigenic drift 2 days ago
- Assessment of CD8+ T-cell mediated immunity in an influenza A(H3N2) human challenge model in Belgium: a single centre, randomised, double-blind phase 2 study 2 days ago
- Dual N-linked glycosylation at residues 133 and 158 in the hemagglutinin are essential for the efficacy of H7N9 avian influenza virus like particle vaccine in chickens and mice 2 days ago
[Go Top] [Close Window]