DUN Ji-feng; ZHOU Ying-chun; PU Juan; LIU Jin-hua. Expression and Analysis of H5N1 Avian influenza virus Hemagglutinin in Baculovirus Expression System. Journal of Agricultural Biotechnology
The hemagglutinin(HA)gene of H5N1 Avian influenza virus(AIV)was amplified from the plasmid pGEM-HA and inserted into Baculovirus transfer plasmid pFastBacHT to construct recombinant transfer vector pFastBacHT-HA.The pFastBacHT-HA was transformed into Escherichia coli DH10Bac competent cells,transposited with Baculovirus shuttle vector(Bacmid)and constructed recombinant transposition rBacmid-HA.After the rBacmid-HA transfected into sf9 cells,the recombinant Baculovirus was harvested.The expressed HA protein ...
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