Jayawardena S, Cheung CY, Barr I, Chan KH, Chen H,. Loop-mediated Isothermal Amplification for Influenza A (H5N1) Virus. Emerg Infect Dis. 2007 Jun; [Epub ahead of print]
We describe a 1-step reverse-transcription loop-mediated isothermal amplification assay for detection of highly pathogenic avian influenza A (H5N1) viruses. The assay was tested by using a panel of highly pathogenic H5N1 subtypes isolated over the past 10 years and clinical specimens. The assay produced negative results for all non-H5N1 subtypes.
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