WEN Kun; QIU Li-wen; WANG Ya-di; CHE Xiao-yan. Expression and identification of H5 subtype hemagglutinin of avian influenza A virus in insect cells. Journal of Southern Medical University
Objective To clone and express avian influenza A virus [A/Hong Kong/482/97(H5N1)] H5 subtype hemagglutinin in baculovirus-insect cell expression system and investigate the antigenicity and bioactivity of the recombinant protein. Methods H5 gene of influenza A virus was amplified by PCR. The recombinant bacmid was obtained by cloning the gene to the donor plasmid of pFastBacHTB and transformed into DH10Bac competent cells. The recombinant baculovirus stock was prepared by transfecting the recombinant bacmid ...
Fulltext in Chinese can be downloaded at Chinese version of F.I.C
See Also:
Latest articles in those days:
- Influenza D Virus Infection in China, 2022-2023 12 hours ago
- Evidence of reassortment of avian influenza A (H2) viruses in Brazilian shorebirds 12 hours ago
- Epitopes in the HA and NA of H5 and H7 avian influenza viruses that are important for antigenic drift 2 days ago
- Assessment of CD8+ T-cell mediated immunity in an influenza A(H3N2) human challenge model in Belgium: a single centre, randomised, double-blind phase 2 study 2 days ago
- Dual N-linked glycosylation at residues 133 and 158 in the hemagglutinin are essential for the efficacy of H7N9 avian influenza virus like particle vaccine in chickens and mice 2 days ago
[Go Top] [Close Window]