WEN Kun; QIU Li-wen; WANG Ya-di; CHE Xiao-yan. Expression and identification of H5 subtype hemagglutinin of avian influenza A virus in insect cells. Journal of Southern Medical University
Objective To clone and express avian influenza A virus [A/Hong Kong/482/97(H5N1)] H5 subtype hemagglutinin in baculovirus-insect cell expression system and investigate the antigenicity and bioactivity of the recombinant protein. Methods H5 gene of influenza A virus was amplified by PCR. The recombinant bacmid was obtained by cloning the gene to the donor plasmid of pFastBacHTB and transformed into DH10Bac competent cells. The recombinant baculovirus stock was prepared by transfecting the recombinant bacmid ...
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