To establish a fluorogenetic quantitative RT-PCR assay for detection of H5N1 tiger influenza A virus (TIV), the HA gene of TIV was aligned using the biologic software and the specific primers were designed in the conserved region of the HA gene by Primer 5.0. For SYBR Green I was cheaper and can be used to distinguish variant of virus, the SYBR Premix Ex TaqTM system was selected. The primers and the reactive condition were optimized to improve the sensitivity and specificity of the assay. The sensitivity ...

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