CHEN Si-huai, etc.,al. CLONING AND ANALYSIS OF HA GENE SEQUENCE OF H6N2 SUBTYPE AVIAN INFLUENZA VIRUS(AIV). Journal of Southwest Agricultural University,2006
With a set of specific primers,the hemagglutinin(HA) gene(1.714 bp in length) of the experimental virulent strain(HB 2002) of avian influenza virus(AIV) was amplified by RT-PCR and successfully cloned to PGEM-T vector.After detecting the cloned sequence and submitting it to Genbank,its accession number was recorded as DQ 285546.The identities of nucleotides and amino acid sequence with Genbank,the motif of cleave sites and the phylogenetic relations were analyzed.The results suggested that the nucleotide an...
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