Highly pathogenic avian influenza A (HPAI) viruses of the H5N1 subtypes caused enormous economical loss to poultry farms in China and Southeastern Asian countries. The vaccination program is a reliable strategy in controlling the prevalence of these disastrous diseases. The six internal genes of the high-yield influenza virus A/Goose/Dalian/3/01 (H9N2),the hemagglutinin (HA) gene of A/Goose/HLJ/QFY/04 (H5N1) strain,and the neuraminidase gene from A/Duck/Germany/1215/73 (H2N3) reference strain were amplified...

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