In order to diagnose quickly tiger influenza in clinic, using the tiger influenza virus (TIV) sequence and the influenza A virus sequence information available in the Genbank data base, the primers were selected from conserved region of a target gene specific for TIV type (NP) and subtype (HA,NA) for use in RT-PCR. Specific primers were desiged for NP, HA, NA gene with Primer5.0 primer design software, DNA fragments of sizes 464, 581 and 173 bp, respectively, were amplified. The primers to be in common used...

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