de Wit E, Bestebroer TM, Spronken MI, Rimmelzwaan. Rapid sequencing of the non-coding regions of influenza A virus. J Virol Methods. 2006 Oct 20; [Epub ahead of print
The non-coding regions (NCRs) of influenza A virus gene segments play a crucial role in the viral replication cycle. Although the NCRs are considered to be conserved, some variation does exist, that affects viral replication. Therefore, a rapid method to sequence the 5´ and 3´ NCRs was designed. This method is based on ligation of viral RNA, RT reactions and subsequent PCR with primersets consisting of a gene segment specific primer and a primer designed across the junction of the 5´ and 3´ ends. These PCR fragments can be sequenced directly without the need for cloning PCR fragments first. This method was used to sequence the NCRs of A/Bilthoven/16190/68 (H3N2) and A/Turkey/Turkey/1/05 (H5N1).
See Also:
Latest articles in those days:
- Epitopes in the HA and NA of H5 and H7 avian influenza viruses that are important for antigenic drift 1 days ago
- Dual N-linked glycosylation at residues 133 and 158 in the hemagglutinin are essential for the efficacy of H7N9 avian influenza virus like particle vaccine in chickens and mice 1 days ago
- Effect of human H3N2 influenza virus reassortment on influenza incidence and severity during the 2017-18 influenza season in the USA: a retrospective observational genomic analysis 1 days ago
- [preprint] Virome Sequencing Identifies H5N1 Avian Influenza in Wastewater from Nine Cities 2 days ago
- [preprint]A systematic review of laboratory investigations into the pathogenesis of avian influenza viruses in wild avifauna of North America 3 days ago
[Go Top] [Close Window]