BAO Hong-mei, etc.,al. Development of a one-step RT-PCR assay for detection of A subtype avian influenza virus. Veterinary Science in China
A one-step RT-PCR assay was developed to detect avian influenza virus(AIV).A pair of specific oligonucleotide primers was designed for the detection of AIV according to M gene sequences.Sensitivity for detection of allantoic fluid by the one-step RT-PCR reached 10 thousand dilutions and detection of swab samples reached 8 dilutions.The tissue and swab samples infected with AIV were simultaneity(detected) by the one-step RT-PCR and the virus isolation method.The sensitivity of the PCR assay was 10 to 100-fol...
Fulltext can be downloaded at Chinese version of FIC
See Also:
Latest articles in those days:
- Highly pathogenic avian influenza as a systemic risk - implications for control and preparedness 17 hours ago
- Multiple introductions and spread of novel reassortant highly pathogenic avian influenza A (H5N1) clade 2.3.4.4b viruses via wild birds, South Korea, 2024-2025 17 hours ago
- [preprint]The mammalian-adaptive PB2-E627K substitution preserves viral fitness of clade 2.3.4.4b H5N1 HPAIV in birds 1 days ago
- Mallard super-shedders of avian influenza exhibit distinct cloacal microbial abundance profiles 1 days ago
- A digitally immune-optimized influenza vaccine broadly neutralizes swine and human H1N1 influenza viruses and protects from heterologous challenge 1 days ago
[Go Top] [Close Window]
