The rationale of this study was to elucidate the etiological agent characteristics and pathogenic mechanisms of the Avian H5N1 influenza virus in mammals,and to offer a platform for evaluating vaccines and drugs against AIV.Male BALB/c mice(18～20g)were anesthetized by inhalation of ether and inoculated intranasally with 0.05mL of infectious virus(A/Tiger/Harbin/01/2002)isolating from a tiger.The dose lethal to 50% of mice(MLD50)was determined by infecting groups of ten with serial 10-fold viral dilutions.Mi...

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