H5N1,a novel avian influenza virus,can infect human directly.In this study,the DNA fragment of H5N1 avian influenza HA gene,antigen determinant,was transferred into tobacco(Nicotiana tabacum cv.) via leaf-discs of Agrobacterium-mediated.Total 38 transgenic plants were regenerated.It was demonstrated that the fragment of H5N1 HA gene had been integrated into the genome of tobacco by PCR and Southern-blotting.The proteins expressed in transgenic plants had immunogenicity by Western-dotting analysis.

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