LI Yao-Chen, ZHAO Zhi-Gang, LI Kang-Sheng. RNA interference mediated inhibition of influenza virus NP and PA gene expressions and multiplication in MDCK cells. Journal of the Fourth Military Medical University
AIM: To apply the small interfering RNAs targeting NP or/and PA gene of influenza virus to inhibit the expression of NP or/and PA and multiplication of influenza virus in Madin-Darby canine kidney (MDCK) cells. METHODS: The recombinant plasmids, pEGFP/NP, pGenesil/PA and pEGFP/NP+ PA were constructed and transfected into MDCK cells. The transfected MDCK cells were infected with subtype H5N1 strain. The transfection efficiency was determined using fluorescence microscopy. The expression levels of NP gene wer...
See Also:
Latest articles in those days:
- T cell help is a limiting factor for rare anti-influenza memory B cells to reenter germinal centers and generate potent broadly neutralizing antibodies 1 days ago
- Wild birds drive the introduction, maintenance, and spread of H5N1 clade 2.3.4.4b high pathogenicity avian influenza viruses in Spain, 2021-2022 1 days ago
- [preprint]FluNexus: a versatile web platform for antigenic prediction and visualization of influenza A viruses 1 days ago
- Salpingitis and multiorgan lesions caused by highly pathogenic avian influenza A(H5N1) virus in a cat associated with consumption of recalled raw milk in California 1 days ago
- Detection of highly pathogenic avian influenza A(H5N1) virus 2.3.4.4b in alpacas 1 days ago
[Go Top] [Close Window]
