The NP gene of the AIV A/Goose/Guangdong/1/96(H5N1) strain was amplified from a recombinant plasmid pMel-NP by PCR and subcloned into an eukaryotic expression vector pVAX1 and the recombinant vector was designated pVAX-NP.The high quality pVAX-NP was prepared and the Hela cells were transfected.The recombinant vector pVAX-NP was successfully expressed in the cells.Indirect immunofluorescence assay and Western-blotting analysis showed that the nucleoprotein was expressed transiently in the Hela cells,which w...