SHI Wen; LU Yi-yu; YAN Ju-ying; et al. TaqMan-based Real-time RT-PCR Assay for Quick Detection of Influenza A1 Virus RNA. Chinese Journal of Vaccines and Immunization
Objective To establish a TaqMan-based real-time PCR assay for detection of influenza A_1 virus RNA.Methods The specific primers and probes were designed in the conserved region of influenza A_1 virus the HA gene.The primers and probes are selected,the reactive condition was and system optimized to improve the sensitivity,specificity and repetition of the assay.The clinical specimens collected from the patients with acute respiratory tract infection were detected by this assay.Results The specificity of the ...
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