Highly pathogenic avian influenza A(H5N1) viruses circulate globally in wild birds, causing poultry outbreaks and spillover infections in mammals and sporadic human infections. Recent detections in domestic cats highlight occupational risks for veterinarians handling cats in areas where A(H5N1) virus is circulating. Although the potential risk for cat-to-human A(H5N1) virus-transmission is currently low, vigilance is warranted. Veterinarians can use published recommendations to evaluate exposure risks, implement infection control practices, use recommended personal protective equipment, train staff, and coordinate with health officials. The One Health approach can help reduce risks of A(H5N1) virus infection to veterinary staff, cat owners, and other animals.