Highly pathogenic avian influenza (HPAI) A(H5N1) and severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are important One Health pathogens with expanding host ranges. We describe an investigation of a bobcat (Lynx rufus) in Washington State in which HPAI H5N1 clade 2.3.4.4b (genotype D1.1) and SARS-CoV-2 were co-detected. Clinical signs, diagnostic findings, and pathology were consistent with H5N1 infection. This report highlights the importance of integrated wildlife surveillance to characterize emerging zoonotic pathogens at the human-wildlife interface.