Influenza viruses can be aerosolized when slaughtering infected chickens, which increases the risk of zoonotic transmission. We conducted pilot experiments to measure the concentrations of airborne particles <2.5 μm during slaughtering and defeathering of chickens to help identify methods that can minimize workers´ exposure to potentially hazardous aerosol particles. By using two types of airborne particle monitors stationed at different heights and angles in a controlled environment, we measured aerosolized particulate matters during exsanguination of 10 slaughtered chickens and use of a mechanical device for defeathering 10 chickens. For the slaughtering experiments, the median particle concentrations at 148 cm height were 67 μg/m3 (IQR 44-121) with a baseline count 10 μg/m3 (IQR 10-10) for the Particle and Temperature Sensor + (PATS+) monitors and 34 μg/m3 (IQR 34-64) with a baseline count 25 μg/m3 (IQR 16-44) for the SidePak? monitor. For the defeathering experiments, the median particle concentrations recorded by the PATS+ monitors were not significantly different between 148 cm (41 μg/m3, IQR 29-49; baseline 12 μg/m3, IQR 10-19) and 107 cm height (37 μg/m3, IQR 29-44; baseline 13 μg/m3, IQR 10-22). Our protocol can be used to test the generation of airborne particles that are <2.5 μm during different slaughtering and defeathering techniques used in the live bird markets to identify procedures that produce the lowest concentrations of small aerosol particles.