Le Y, Zhang J, Gong Z, Zhang Z, Nian X, Li X, Yu D. TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus. Heliyon. 2023 Aug 21;9(9):e19246
Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus (IVA) remains elusive. In the present study, CRISPR-Cas9 gene editing technology was used to construct the TRAF3 knockout MDCK cells (MDCK-TRAF3-/-). Hemagglutination assay, plaque assay, transcriptome, and quantitative real-time PCR were performed after IVA infection. The results showed that after IVA infection, HA titers and virus titers were promoted, interferon I-related pathways were significantly blocked, and transcription of several antiviral-related genes was significantly decreased in MDCK-TRAF3-/- cells. Thus, our study suggests that TRAF3 gene knockout reduced MDCK cell´s resistance to IVA, thereby resulting in a promising way for IVA isolation and vaccine manufacturing.
See Also:
Latest articles in those days:
- Phylogeography and gene pool analysis of highly pathogenic avian influenza H5N1 viruses reported in India from 2006 to 2021 1 days ago
- Analysis of a diffusive epidemic model with a zero-infection zone 1 days ago
- Quick detection of H5N1 avian influenza virus by surface enhanced Raman scattering(SERS) using aptamer capture 1 days ago
- The critical role of RAGE in severe influenza infection: A target for control of inflammatory response in the disease 1 days ago
- Human infection caused by avian influenza A (H10N5) virus 1 days ago
[Go Top] [Close Window]