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2022-8-9 21:49:19

Sewald K, Danov O. Infection of Human Precision-Cut Lung Slices with the Influenza Virus. Methods Mol Biol. 2022;2506:119-134
submited by kickingbird at Jul, 1, 2022 17:28 PM from Methods Mol Biol. 2022;2506:119-134

Viral infections are common causes of asthma exacerbations. To model these processes ex vivo, human precision-cut lung slices (PCLSs) can be used. Here we describe the infection of human PCLSs with the human influenza virus. We then provide methods to quantify the virus and reveal its localization within infected PCLSs and study consequences of infection, including cell death and production of cytokines, chemokine, and mucus. We also describe the stimulation of PCLSs with immune mediators such as pro-inflammatory tumor necrosis factor α (TNF-α). These models are useful to investigate mechanisms of virally induced asthma exacerbations and study modes of action and efficacy of antiviral and/or anti-inflammatory drugs.

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