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2020-11-24 6:30:16


Yang F, Xu L, Liu F, Yao H, Wu N, Wu H. Development and evaluation of a TaqMan MGB RT-PCR assay for detection of H5 and N8 subtype influenza virus. BMC Infect Dis. 2020;20(1):550
submited by kickingbird at Jul, 31, 2020 11:47 AM from BMC Infect Dis. 2020;20(1):550

Background: Highly pathogenic influenza A (H5N8) viruses have caused several worldwide outbreaks in birds and are of potential risk to humans. Thus, a specific, rapid and sensitive method for detection is urgently needed.
Methods: In the present study, TaqMan minor groove binder probes and multiplex real-time RT-PCR primers were designed to target the H5 hemagglutinin and N8 neuraminidase genes. A total of 38 strains of avian influenza viruses and other viruses were selected to test the performance of the assay.
Results: The results showed that only H5 and N8 avian influenza viruses yielded a positive signal, while all other subtypes avian influenza viruses and other viruses were negative. High specificity, repeatability, and sensitivity were achieved, with a detection limit of 10 copies per reaction.
Conclusions: The developed assay could be a powerful tool for rapid detection of H5N8 influenza viruses in the future.

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