Kombiah S, et al. Experimental pathology of two highly pathogenic H5N1 viruses isolated from crows in BALB/c mice. Microb Pathog. 2020 Jan 20:103984.
In our study we assessed the pathogenicity of two H5N1 viruses isolated from crows in mice. Eighteen 6-8 weeks BALB/c mice each were intranasally inoculated with 106 EID50/ml of H5N1 viruses A/crow/India/03CA04/2015 (H9N2-PB2 reassortant H5N1) and A/crow/India/02CA01/2012 (Non-reassortant H5N1). The infected mice showed dullness, weight loss and ruffled fur coat. Histopathological examination of lungs showed severe congestion, haemorrhage, thrombus, fibrinous exudate in perivascular area, interstitial septal thickening, bronchiolitis and alveolitis leading to severe pneumonic changes and these lesions were less pronounced in reassortant virus infected mice. Viral replication was demonstrated in nasal mucosa, lungs, trachea and brain in both the groups. Brain, lung, nasal mucosa and trachea showed significantly higher viral RNA copies and presence of antigen in immunohistochemistry in both the groups. This study concludes that both the crow viruses caused morbidity and mortality in mice and the viruses were phenotypically highly virulent in mice. The H5N1 viruses isolated from synanthropes pose a serious public health concern and should be monitored continuously for their human spill-over.
See Also:
Latest articles in those days:
- Abundant Intra-Subtype Reassortment Revealed in H13N8 Influenza Viruses 2 hours ago
- Locations and structures of influenza A virus packaging-associated signals and other functional elements via an in silico pipeline for predicting constrained features in RNA viruses 6 hours ago
- Molecular Characterization of Non-H5 and Non-H7 Avian Influenza Viruses from Non-Mallard Migratory Waterbirds of the North American Flyways, 2006~2011 12 hours ago
- A case report and literature review on tocilizumab-cured acute necrotizing encephalopathy caused by influenza A virus 12 hours ago
- Study on the clinical efficacy and safety of baloxavir marboxil tablets in the treatment of influenza A 13 hours ago
[Go Top] [Close Window]