The HA gene of tiger influenz a A virus (A/Tiger/Harbin/01/2003) was cloned and analyzed. The ge netic analysis of HA gene revealed that the HA gene of the virus had a lo ng complete ORF composed of 1070 nucleotides, coding 568 amino acids. The HA gen e had a high homology with the H5 gene of influenza A virus. Moreover the HA gen e displayed polybasic amino acids at cleavage site, which indicates a highly pathogenic avian influenza. The HA gene was cloned into baculovirus expression vectors pFastBacâ… , and ...
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